HisLink Protein Purification Resin from Promega

Product Specs
ItemHisLink Protein Purification Resin
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV8823
Quantity5ml
Product TypeResins
ModeAffinity Purification, Metal Chelate
Applicationspurification

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Enzyme stabilization and thermotolerance function of the intrinsically disordered LEA2 proteins from date palmScientific ReportsJuly 23, 2023Mughair Abdul Aziz, Miloofer Sabeem, M. Sangeeta Kutty, Shafeeq Rahman, Maitha Khalfan Alneyadi, Alia Binghushoom Alkaabi, Eiman Saeed Almeqbali, Faical Brini, Ranjit Vijayan, Khaled Masmoudiprotease inhibitor phenylmethylsulfonyl fluoride (PMSF). Protein purification was performed by using the HisLink Protein Purification Resin according to the manufacturer instructions (Promega). By using SDS-PAGE, five μg of protein from each

(2) Cooperative Regulation of Flagellar Synthesis by Two EAL-Like Proteins upon Salmonella Entry into Host CellsMicrobiology SpectrumApril 13, 2023Yingying Yue, Weiwei Wang, Yue Ma, Nannan Song, Haihong Jia, Cuiling Li, Qi Wang, Hui Li, Bingqing Liusing the Maxquant search engine (v.1.5.2.8). Pulldown assay. FlhD4C2 with a His tag was mixed with HisLink protein purification resin (Promega, Madison, WI) in the presence or absence of STM1344 and mutants without the His tag. After

(3) Formation of synthetic RNA protein granules using engineered phage-coat-protein -RNA complexesNat CommunNovember 10, 2022Naor Granik, Noa Katz, Or Willinger, Sarah Goldberg, Roee Amitextract. Fusion protein was purified using HisLink Protein purification resin (Promega (Madison, WI) #V8821) according to the manufacturer’s instructions. Buffer was changed to 1xPBS (Biological Industries, Israel)

(4) The N Terminus of Adhesion G Protein–Coupled Receptor GPR126/ADGRG6 as Allosteric Force IntegratorFrontiers in Cell and Developmental BiologyJune 23, 2022Jakob Mitgau, Julius Franke, Camilla Schinner, Gabriele Stephan, Sandra Berndt, Dimitris G. Placantonakis, Hermann Kalwa, Volker Spindler, Caroline Wilde, Ines Liebscherwas collected and purification was carried out via His-tag using HisLink Protein Purification Resin (V8823, Promega) according to the manufacturer’s instructions. Protein purity was confirmed by Western blot

(5) Staphylococcus aureus β-Toxin Exerts Anti-angiogenic Effects by Inhibiting Re-endothelialization and Neovessel FormationFrontiers in microbiologyJanuary 1, 2022Tran PM, Tang SS, Salgado-Pabón W.LAL Endotoxin Assay Kit (L00350) was purchased from Genscript. HisLink™ Protein Purification Resin (V8823) and CellTiter 96® AQueous One Solution (G3582) were purchased from Promega. Mitomycin C (M4287) was

(6) Identification of Binding Regions of Bilirubin in the Ligand-Binding Pocket of the Peroxisome Proliferator-Activated Receptor-A (PPARalpha)MoleculesMay 17, 2021Darren M. Gordon, Stephen H. Hong, Zachary A. Kipp, Terry D. Hindsvia centrifugation and resuspended in 200 µM HEPES. The His-Tagged proteins were extracted using the HisLink™ Protein Purification Resin (Promega, Madison, WI, USA). Proteins were subsequently dialyzed overnight to remove excess elution

(7) Tick Importin-Î± Is Implicated in the Interactome and Regulome of the Cofactor SubolesinPathogensApril 11, 2021Sara Artigas-JerÃ³nimo, Margarita Villar, Alejandro Cabezas-Cruz, GrÃ©gory Caignard, Damien Vitour, Jennifer Richardson, Sandrine Lacour, Houssam Attoui, Lesley Bell-Sakyi, Eleonore Allain, et al.and 10 mM imidazole (binding and wash buffer) for 1 h with shaking, at a concentration ratio of 2:1. HisLink Protein Purification Resin (Promega Corporation, Madison, WI, USA) was washed following the manufacturer’s recommendations and blocked

(8) PIM-induced phosphorylation of Notch3 promotes breast cancer tumorigenicity in a CSL-independent fashionJournal of Biological ChemistryMarch 26, 2021Sebastian K.J. Landor et al.lysis buffer. For immunoprecipitation of His-tagged PIM1, 500 μg of protein was combined with 50 μl of HisLink Protein Purification Resin (Promega) in 500 μl of lysis buffer supplemented with 10 mM imidazole (104716; Merck). Samples were

(9) Exoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated Staphylococcus aureus ST398 strainsVirulenceDecember 31, 2020Xin Zhao, Monika A. Chlebowicz-Flissikowska, Min Wang, Elias Vera Murguia, Anne de Jong, DÃ¶rte Becher, Sandra MaaÃŸ, Girbe Buist, Jan Maarten van Dijlculturing at 30°C, and the His6-tagged proteins were purified from the supernatant fractions using HisLink™ Protein Purification Resin (Promega Corporation, Madison, WI. USA). Lastly, the three purified proteins were analyzed by LDS-PAGE

(10) Radiolabeled cCPE Peptides for SPECT Imaging of Claudin-4 Overexpression in Pancreatic CancerJournal of Nuclear MedicineDecember 1, 2020Julia Baguña Torres, Michael Mosley, Sofia Koustoulidou, Samantha Hopkins, Stefan Knapp, Apirat Chaikuad, Masuo Kondoh, Keisuke Tachibana, Veerle Kersemans, Bart Cornelissenβ-d-1-thiogalactopyranoside (MP Biomedicals LLC) addition and purified by affinity chromatography using HisLink protein purification resin (Promega). A detailed procedure is laid out in the supplemental data. Purified proteins were dialyzed

(11) An optimized desuccinylase activity assay reveals a difference in desuccinylation activity between proliferative and differentiated cellsScientific ReportsDecember 1, 2020Taolin Yuan, Jaap Keijer, Angela H. Guo, David B. Lombard, Vincent C. J. de Boerfinal concentration of 30 mM. Crude His-SIRT5 was purified using HisLink protein purification resin (#V8823, Promega) in combination with a chromatography column (#731-1150, Bio-Rad). The protein purification

(12) Structural properties and enzyme stabilization function of the intrinsically disordered LEA_4 protein TdLEA3 from wheatScientific ReportsDecember 1, 2019Sana Koubaa, Anne Bremer, Dirk K. Hincha, FaiÃ§al BrinimM Tris-HCl, pH 7.9) containing the protease inhibitor phenylmethylsulfonyl fluoride and purified by HisLink Protein Purification Resin according to the manufacturer’s (Promega) instructions. The concentration of the recombinant protein

(13) Distinct structural classes of activating FOXA1 alterations in advanced prostate cancerNatureJuly 1, 2019Parolia A, Cieslik M, Chu SC, Xiao L, Ouchi T, Zhang Y, Wang X, Vats P, Cao X, Pitchiaya S, et al.glycerol) in the presence of protease inhibitors (Roche). HisLink Purification Resin (Promega, Cat#: V8821) was used to purify untagged recombinant proteins from the crude bacterial lysates as per manufacturer’s

(14) There's no place like OM: Vesicular sorting and secretion of the peptidylarginine deiminase of Porphyromonas gingivalisVirulenceDecember 31, 2018Giorgio Gabarrini, Laura M. Palma Medina, Tim Stobernack, Rianne C. Prins, Marines du Teil Espina, Jeroen Kuipers, Monika A. Chlebowicz, John W. A. Rossen, Arie Jan van Winkelhoff, Jan Maarten van Dijllactis) or bead-beating (E. coli) in binding buffer (S3 Table). Cell lysates were added to 500 µL of HisLink™ Protein Purification Resin (Promega, USA) and incubated at room temperature for 30–60 min. To remove non-specifically bound proteins,

(15) HU protein is involved in intracellular growth and full virulence ofFrancisella tularensisVirulenceDecember 31, 2018Pavla Stojkova, Petra Spidlova, Juraj Lenco, Helena Rehulkova, Lucie Kratka, Jiri Stulikpsi). Purification of FtHU was done with Amicon Pro Affinity Concentrator (Merck Millipore, ACK5003PG) and HisLink Protein Purification Resin (Promega, V882A). The sample was mixed with resin and incubated at 4°C for 30 min. Proteins unbound

(16) Amyloid Aggregation of Bacillus circulans Xylanase under Native Conditions and its Modulation by β‐Amyloid‐Derived Peptide FragmentsChemBioChemDecember 18, 2018Timothy Charlton, Vandan Shah, Tonianna Lynch, Jason Candreva, Edward Chau, YanXi Yang, Hyunjoo Kim, Amy Wood, Jin Ryoun Kimresidues (D1-V40; referred to as A40) was purchased from the ERI Amyloid Laboratory (Oxford, CT, USA). HisLink Protein Purification Resin was purchased from Promega (Madison, WI, USA). A HiPrep 16/60 Sephacryl S-100 column and other FPLC

(17) In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteriaBiotechnology for BiofuelsDecember 1, 2018Elrike Frenzel, Jelmer Legebeke, Atze van Stralen, Richard van Kranenburg, Oscar P. Kuipersinstructions. The His6-tag and uncleaved proteins still containing the His6-tag were removed with the HisLink Protein Purification Resin (Promega) according to the manufacturer’s protocol. For in vitro characterization of FP properties,

(18) Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteinsScientific ReportsDecember 1, 2018Francisco Romero Pastrana, Jolanda Neef, Dennis G. A. M. Koedijk, Douwe de Graaf, José Duipmans, Marcel F. Jonkman, Susanne Engelmann, Jan Maarten van Dijl, Girbe BuistSubsequently, his-tagged proteins were purified from the respective supernatant fractions using the HisLink Protein Purification resin (Promega Corporation, Madison, WI. USA), in the absence or presence of either 2 M KSCN or 6 M urea.

(19) Pancreatic Î²-cell tRNA hypomethylation and fragmentation link TRMT10A deficiency with diabetesNucleic Acids ResearchNovember 2, 2018Cristina Cosentino, Sanna Toivonen, Esteban DiazÂ Villamil, Mohamed Atta, Jean-Luc Ravanat, StÃ©phane Demine, AndreaÂ Alex Schiavo, Nathalie Pachera, Jean-Philippe Deglasse, Jean-Christophe Jonas, et al.pET30a-AlkB-D135S Addgene plasmid # 79051). The recombinant His-tagged proteins were purified using HisLink protein purification resin (Promega). After treatment, the RNA samples were polyadenylated and reverse transcribed using miRCURY

(20) Selective terminal methylation of a tRNA wobble baseNucleic Acids ResearchApril 20, 2018Isao Masuda, Ryuichi Takase, Ryuma Matsubara, Mellie June Paulines, Howard Gamper, Patrick A Limbach, Ya-Ming Hou4 h at 37°C in LB containing 0.1 mM IPTG. The dual 6xHis-tagged recombinant CmoM was purified with HisLink Protein Purification Resin (Promega) as described previously (5). The recombinant E. coli TrmD was prepared as described (27–29).

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